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DiaPharma is a distributor of test kits, bioreagents, chromogenic substrates, antibodies and instruments in the fields of Thrombosis and Hemostasis (Coagulation and Fibrinolysis), and Apoptosis. Our clinical test kits are used in hospital laboratories, clinical reference laboratories and universities. Our line of research products is marketed to researchers and medical corporations for use in product development and in quality control laboratories. |
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NEW! Technozym ADAMTS-13 Activity ELISA!
Advantages of Technozym ADAMTS-13 Activity:
- ADAMTS-13 activity sensitivity down to 0.5% of normals
- Sensitivity of measurement for inhibitors
- No need for any special technique or instrument, just standard ELISA equipment
- Short assay time (3h)
- Calibrators and controls included
New! Application for the BCS now available!
If you're still using a method based on antithrombin's ability to inhibit thrombin, now's your chance to try a better method. For a limited time, DiaPharma is offering a free evaluation Coamatic Antithrombin kit to new customers. Coamatic Antithrombin is a simple, accurate, and convenient assay based on FXa inhibition. The FXa-based assay has been shown to discriminate better between AT-deficient and non-AT-deficient individuals than a thrombin-based assay, without interference from heparin cofactor II or direct thrombin inhibitors.
Features of Coamatic Antithrombin:
- Use of FXa instead of thrombin eliminates interference from heparin cofactor II anddirect thrombin inhibitors
- Highly sensitive FXa chromogenic substrate and superior reagent stability
- High accuracy and precision - CV values less than 5%
- Applications available for a wide range of automated coagulation analyzers, including the BCS
- Accurate determinations in patients receiving heparin therapy
- Suitable for both large and small laboratories
Focus Products
DiaPharma presents our newest innovative products for measurement of microparticle activity
Quantitative measurement of procoagulant activity of microparticles on citrated plasma - a convenient functional assay in a 96 well microplate format.
Microparticles present in plasma bind to Annexin V that has been immobilized in microwells and then expose their phospholipid surface. This allows FXa-FVa, in the presence of calcium, to activate prothrombin to thrombin, which is detected using a thrombin sensitive chromogenic substrate. The amount of pro-coagulant activity due to the presence of microparticles is proportional to the amount of thrombin generated.
- First quantitative functional assay
- Reagent-complete kit with calibrators and controls, in a convenient microplate format
- Optimized for routine use
- Results in 90 minutes
Easy to use thrombin generation assay for measuring thrombotic activity of procoagulant microparticles
Procoagulant microparticles, generated by activation of blood cells such as platelets, endothelial cells, and leucocytes can be observed at high levels in certain pathological conditions. Circulating microparticles contribute significantly to thrombus formation and are thought to be one of the major risk factors for thrombosis in atherosclerotic patients. DiaPharma introduces two assays for the quantitative measurement of microparticle activity.
Technothrombin® TGA is a thrombin generation assay (TGA) based on monitoring the formation of thrombin by means of a fluorogenic substrate upon activation of the coagulation cascade by tissue factor.
- Allows for direct relation of circulating microparticle levels to microparticle-induced thrombotic tendency
- Measure thrombogenic microparticles generated during endotoxinemia
- Determine thrombogenic activity of apoptotic microparticles
- Analyze mechanisms leading to thrombin generation induced by these apoptotic microparticles
Read the JAMA article and commentary.
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On Sale Now
60% Off:
- PP001A (Human Fibrinogen)
While supplies last. Select lot(s) only. Sale ends May 31, 2008. Call 1-800-526-5224 for details.
50% Off:
While supplies last. Select lot(s) only. Sale ends June 31, 2008. Call 1-800-526-5224 for details.
30% Off:
While supplies last. Select lot(s) only. Sale ends July 30, 2008. Call 1-800-526-5224 for details.
Surveys
Please click here for our Coagulation Analyzer Survey! - the first 100 qualified hemostasis professionals receive a free lunch bag.
Tech Tips
When performing Coatest® APC Resistance and Coatest® APC Resistance V/VS, pay attention to the clotting times as well as your ratio. The baseline APTT time (only CaCl2 ), which is the bottom number in your ratio, should be within the range of APTT times you observed when you established when determining your cut-off. Typical values are about 30 - 45 seconds, and will vary between labs and instrumentation.
Although the 1:4 pre-dilution with Factor V Deficient Plasma strongly decreases interferences, prolonged baseline APTT may occur in patient plasmas with high inhibitor activity (e.g. phospholipid antibodies). In that case, increasing the dilution (e.g. 1:9 or 1:19) may correct the result. If the additional dilution with Factor V Def Plasma does not correct the clotting time, then the calculated APC ratio is not valid. It is important to flag any results from patient plasmas with a baseline APTT time that lies outside of your normal distribution to avoid misleading APC ratio results.
Got a tech question about any of our products? E-mail jkiblinger@diapharma.com or call 800.447.3846.
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