For Research Use Only. Not For Use In Diagnostic Procedures.

Reagents -- Composition, Packaging and Storage

1. Capture Antibody (Catalog No. PCI-EIA-C): Yellow capped tube containing 0.5 ml of affinity purified goat anti-Protein C Inhibitor (PCI) IgG for coating plates.
2. Detecting Antibody (Catalog No. PCI-EIA-D): Red capped tube containing 0.5 ml of peroxidase labelled anti-Protein C Inhibitor for detection of PCI complexes.

Both reagents are supplied in 50% glycerol and should be stored at -20°C.

Assay Procedure

Coating of plates: Dilute the capture antibody 1/100 in coating buffer and immediately add 100 microliters to every well in the microplate. Incubate overnight @ 4°C.

Blocking: Empty contents of plate and add 150 microliters of blocking buffer to every well and incubate for a minimum of 60 minutes @ 22°C. Wash plate X4 with PBS-Tween.

Samples: Dilutions of standard normal plasma are made in a range from 1/100 (100%) down to 1/3200 (3.13%). Test plasma samples are diluted 1/200 & 1/400. All dilutions are made in HBS-BSAT20. Apply 100 microliters/well and incubate plate @ 22°C for 90 minutes. Wash X 4 with PBS-Tween.

Detecting Antibody: Dilute the detecting antibody 1/100 in HBS-Tween-BSA and apply 100 microliters to every well. Incubate plate @ 22°C for 90 minutes. Wash X 4 with PBS-Tween.

OPD Substrate: Apply 100 microliters of freshly prepared OPD substrate to every well. Allow color to develop for 5-10 minutes then stop color reaction with the addition of 50 microliters/well of 2.5 M H2SO4.

Related products:

• GAPCI-IG - Goat anti-human PCI, purified whole IgG from antiserum 10 mg vial
• GAPCI-AP - Goat anti-human PCI, affinity purified IgG 0.5 mg vial
• GAPCI-HRP - Goat anti-human PCI, peroxidase labelled whole IgG 0.2 mg vial
• PCI-DP - Human plasma deficient in PCI, immune depleted, frozen 0.5 ml vial