Catalog #: K823120 (823120)
An APTT-based kit for the screening of factor-V-related APC resistance. The high sensitivity and specificity of the test for the factor V:Q506 mutation is obtained by prediluting the sample plasma with an excess of V-DEF Plasma bioreagent. The test design makes it possible to discriminate between heterozygous and homozygous factor V genotypes. It also allows for analysis of plasma from patients on heparin or oral anticoagulant therapy. High discrimination between genotypes with 100% sensitivity for FV:Q506. Reduces need for PCR determination. Applicable to anticoagulant treated patients.
Measurement principle
One volume of plasma is prediluted with four volumes of V-DEF Plasma. The dilution is then incubated with the APTT reagent for a standard period of time. Coagulation is triggered by the addition of CaCl2 in the absence and presence of exogenous APC and the time for clot formation is recorded.
Reagents and Stability when opened
|
Reagent |
Units APC V |
Units APC VS |
Units APC |
Expiration |
Temperature |
|
V-DEF Plasma |
4 × 4 mL |
2 × 4 mL |
n/a |
8 hours |
15-25°C |
|
24 hours |
2-8°C |
||||
|
3 months |
-20°C |
||||
|
CaCl2 |
1 × 8 mL |
2 × 2 mL |
2 × 2 mL |
1 week |
15-25°C |
|
1 month |
2-8°C |
||||
|
APTT Reagent |
1 × 16 mL |
2 × 4 mL |
2 × 4 mL |
1 week |
15-25°C |
|
1 month |
2-8°C |
||||
|
APC/CaCl2 |
4 × 2 mL |
2 × 2 mL |
2 × 2 mL |
8 hours |
15-25°C |
|
5 days |
2-8°C |
||||
|
3 months |
-20°C |
||||
|
Control level 1 |
1 × 1 mL |
1 × 1 mL |
n/a |
6 hours |
15-25°C |
|
3 months |
-20°C |
||||
|
Control level 2 |
1 × 1 mL |
1 × 1 mL |
n/a |
6 hours |
15-25°C |
|
3 months |
-20°C |
||||
| Name | Type | Size |
|---|---|---|
| Chromogenix Coamatic / Coatest Thrombophilia Flyer | application/pdf | 536.13 kB |
| Chromogenix Coatest APC Resistance Brochure | application/pdf | 107.78 kB |
| Chromogenix Coatest APC Resistance Flyer | application/pdf | 182.93 kB |
| Chromogenix Coatest APC Resistance Monograph | application/pdf | 527.75 kB |
| Chromogenix Coatest APC Resistance V Material Safety Data Sheet (MSDS) | application/pdf | 125.44 kB |
| Chromogenix Coatest APC Resistance V Package Insert | application/pdf | 109.83 kB |
| Name | Type | Size |
|---|---|---|
| APC Resistance ISTH Satellite Symposium Invitation 1997 (old) | application/pdf | 1.37 MB |
| APC Resistance ISTH Satellite Symposium Program & Abstracts 1997 (old) | application/pdf | 118.54 kB |
| Coatest APC Resistance Product Range 1996 (old) | application/pdf | 5.35 MB |
| Coatest APC Resistance V Flyer 1997 (old) | application/pdf | 3.55 MB |
| Coatest APC Resistance V Flyer 1998 (old) | application/pdf | 2.84 MB |