Buffered bentonite suspension, ready to use; contains 0.9 g/L Sodium Azide as preservative.

Use:

Removal of Fibrinogen from plasma for testing Fibrino Peptide A (FPA). Mix thoroughly before use. When opened, it can be used for 4 weeks, stored at 2-8°C, and provided that contamination is avoided.

Treatment of plasma:

Before measurement of FPA, cross reactive Fibrinogen must be removed from plasma by bentonite adsorption. Mix thoroughly the bentonite suspension in order to make it homogeneous. To 1 ml of the anticoagulated plasma (special anticoagulant for FPA testing), add 0.5 ml of bentonite suspension. Mix and agitate for 10 min. using an end-over-end agitator. Centrifuge for 20 min. at 2,500 g and collect 1 ml supernatant.

Proceed to a new bentonite adsorption by adding again 0.5 ml of suspension to the 1 ml of supernatant, in a similar manner. The bentonite treated plasma is then fibrinogen free. It must be used within 24 hours at room temperature (2-8°C) or 1 month when stored frozen at -20°C or below.

The bentonite treated plasma is two-fold diluted, and the measured FPA concentration must be multiplied by 2.