Research ELISA for determination of von Willebrand Factor (vWF) antigen in plasma and concentrates. In combination with the vWF:CBA ELISA Kit classification of von Willebrand disease type 1, 2 and 3 is possible. Assay time is only 60 minutes.

COMPOSITION

1. ELISA test strips (12) with 8 wells each, coated with polyclonal anti-vWF; the drying agent is supplied in an aluminum bag.
2. Washing buffer concentrate: (PBS; pH 7.3); containing detergent; 0.01% merthiolate; 1 bottle, 100 ml.
3. Incubation buffer: (PBS; pH 7.3); contains stabilizer protein; 0.01% merthiolate; dye, 1 bottle, 100 ml, ready for use.
4. Calibrators (Standards) numbered; lyophilized; 1 bottle each. Concentrations are lot-dependent; consult label on the vial.
5. Control plasmas "low level" and "high level" for checking purposes, lyophilized; 1 bottle each. Concentrations are lot-dependent; consult the label on the vial.
6. Conjugate polyclonal Anti-vWF-POX; dyed blue; 1 bottle, 0.3 ml.
7. Chromogen TMB (tetramethylbenzidine); 1 bottle, 12 ml; ready to use.
8. Stopping solution: sulphuric acid 1.9 mol/l; 1 bottle 12ml; ready for use.
9. Adhesive film: for ELISA test strips (2)