What is the best concentration to use these antibodies for ELISA, immunoblotting and immunohistochemistry applications?

The optimal concentration of antibody for any particular application will vary depending on the antibody format as well as the detection method used. Affinity Biologicals strongly suggests that each antibody be titrated by the end user to determine the optimal working concentration for each particular application. However, based on experience, Affinity Biologicals has made some general recommendations regarding antibody concentration ranges that can be used as a starting point in optimizing assay conditions. The following chart contains general recommendations only and may not apply to all our Affinity Biologicals products.