Apoptosis & Apoptosense

I have a very large number of samples to test, and I don't want to mess with a complicated assay.

The ELISA format is a simple way to examine a large number of samples in one day. Drug candi...

I need an assay that is sensitive and robust.

The M30-Apoptosense® assay is very sensitive, as it easily detects apoptosis in less than 5...

Briefly describe the procedure for the M30-Apoptosense assay.

The assay is a solid-phase, two-site immunosorbent assay. Samples are reacted with polystyre...

Can all cell types be used?

Only epithelial derived cells, not fibroblasts, lymphocytes, or other cells that do not expr...

Can both serum and plasma samples be used?

Yes, the levels (U/L) are similar but not identical. For serial measurements it is recommend...

Can components from different lot numbers be mixed?

No, do not mix components from different lots.

Does hemolysis affect the levels of M30-Apoptosense measurements in a sample?

No, normally hemolysis does not interfere.

Does the assay measure caspase activity? What is the advantage or disadvantage to what it measures?

No, the M30-Apoptosense® assay does not measure caspase activity. The assay instead measure...

Does the M30-Apoptosense assay also measure necrosis?

No. Due to the specificity of the M30 antibody to a caspase cleavage product, it only recogn...

How should samples be stored?

Cell extracts should be frozen at -20°C immediately. Serum samples can be stored for 48 hou...

How specific is the assay with other species?

Caspase-cleaved cytokeratin 18 from human, rat, mouse and rabbit can be recognized.

MCF-7 cells are known to be defective in caspase-3 activity. Can I still use the M30-Apoptosense assay?

Yes, more than one caspase can generate the M30 neo-epitope. MCF-7 cells generate M30-activi...

The method I am using now does not distinguish between apoptotic, necrotic, and viable cells. How is the M30-Apoptosense different?

Some methods, such as TUNEL (DNA labeling), do not discern necrotic and apoptotic cells. Sin...

The methods I use measure the late stages of apoptosis. How is this product different?

The M30-Apoptosense® assay measures early apoptosis, as opposed to methods that measure DNA...

What can the M30-Apoptosense assay be used for?

The M30-Apoptosense® is an ELISA for the quantitative measurement of the apoptosis-associat...

What does the M30-Apoptosense assay contribute that the Caspase-3 assay, or other caspase assays, does not?

The assays measure different processes. The Caspase-3 assay measures enzyme activity while t...

What is the advantage of M30-Apoptosense relative to other methods?

M30-Apoptosense® is a simple 96 well format, quantitative rapid screening test. It is conve...

What is the measurement theory behind the M30-Apoptosense assay?

During apoptosis, caspases will cleave various cellular proteins. In epithelial cells, one o...

What kind of samples can be analyzed?

M30-antigen released from epithelial cells (culture supernatants), antigen present in cell e...