DiaPharma Group, Inc.

For Research Use Only. Not For Use In Diagnostic Procedures.

1. Capture Antibody (Catalog No. FVII-EIA-C): One yellow-capped vial containing 0.5 ml of purified monoclonal anti-FVII antibody for coating plates.
2. Detecting Antibody (Catalog No. FVII-EIA-D): One red-capped vial containing 0.5 ml of peroxidase-conjugated polyclonal anti-FVII antibody for detection of captured FVII.

Note: Antibodies are supplied in a 50% (v/v) glycerol solution for storage at -10 to -20°C. Keep vials tightly capped. Do not store in frost-free freezers.

Principle of Sandwich-style ELISA

Affinity-purified antibody to FVII is coated onto the wells of a microtitre plate. Remaining binding sites on the plate are blocked with an excess of bovine albumin. The plates are washed and plasma or other fluids containing FVII are applied. The coated antibody will capture the FVII in the sample. After washing the plate to remove unbound material, a peroxidase conjugated second antibody to FVII is added to the plate to bind to the captured FVII. After washing the plate to remove unbound conjugate, the peroxidase activity is expressed by incubation with ophenylenediamine (OPD). After a fixed development time the reaction is quenched with the addition of H2SO4 and the color produced is quantified using a microplate reader. The color generated is proportional to the concentration of FVII present in the sample.

Related Products:

• SAFVII-IG - Sheep anti-human FVII, IgG from antiserum
• SAFVII-AP - Sheep anti-human FVII, affinity purified IgG
• SAFVII-HRP - Sheep anti-human FVII, IgG-peroxidase conjugate
• FVII-DP - Plasma deficient in FVII, immune depleted