DiaPharma Group, Inc.

For Research Use Only. Not For Use In Diagnostic Procedures.

Reagents -- Composition, Packaging and Storage

1. Capture Antibody (Catlog No. F8C-EIA-C): One yellow-capped vial containing 0.4 ml of polyclonal affinity purified anti-FVIII antibody for coating plates.
2. Detecting Antibody (Catlog No. F8C-EIA-D): Four neutral-capped tubes each containing 10 ml of pre-diluted peroxidase conjugated polyclonal anti-FVIII antibody for detection of captured FVIII.
3. Sample Diluent (Catlog No. F8C-EIA-SD): 100 ml bottle containing a green-coloured diluent optimised for dilution of samples.

Store reagents at 2-8°C

Principle of Sandwich-style ELISA

Affinity-purified antibody to FVIII is coated onto the wells of a microtitre plate. The plate is washed and plasma or other fluids containing FVIII are applied. The coated antibody will capture the FVIII in the sample. After washing the plate to remove unbound material, a peroxidase conjugated second antibody to FVIII is added to the plate to bind to the captured FVIII. After washing the plate to remove unbound conjugated antibody, the peroxidase activity is expressed by incubation with o-phenylenediamine (OPD). After a fixed development time the reaction is quenched with the addition of H2SO4 and the color produced is quantified using a microplate reader. The color generated is proportional to the concentration of FVIII present in the sample.

Related Products:

• SAF8C-IG - Sheep anti-human Factor VIII, IgG from antiserum
• SAF8C-AP - Sheep anti-human Factor VIII, affinity purified IgG
• SAF8C-HRP - Sheep anti-human Factor VIII, whole IgG-peroxidase
• FVIII-DP - Human plasma deficient in FVII, immune depleted