DiaPharma Group, Inc.

For Research Use Only. Not For Use In Diagnostic Procedures.

Reagents -- Composition, Packaging and Storage

1. Capture Antibody (Catalog No. FX-EIA-C): One yellow-capped vial containing 0.5 ml of affinity-purified polyclonal anti-FX antibody for coating plates.
2. Detecting Antibody (Catalog No. FX-EIA-D): One red-capped vial containing 0.5 ml of peroxidase conjugated polyclonal anti-FX antibody for detection of captured FX.

Note: Antibodies are supplied in a 50% (v/v) glycerol solution for storage at -10 to -20°C. Keep vials tightly capped. Do not store in frost-free freezers.

Principle of Sandwich-style ELISA

Affinity-purified polyclonal antibody to FX is coated onto the wells of a microtitre plate. Any remaining binding sites on the plastic wells are blocked with an excess of bovine serum albumin. The plates are washed and plasma or other fluids containing FX are applied. The coated antibody will capture the FX in the sample. After washing the plate to remove unbound material, a peroxidase conjugated second antibody to FX is added to the plate to bind to the captured FX. After washing the plate to remove unbound conjugated antibody, the peroxidase activity is expressed by incubation with o-phenylenediamine (OPD). After a fixed development time the reaction is quenched with the addition of H2SO4 and the color produced is quantified using a microplate reader. The color generated is proportional to the concentration of FX in the sample.

Related Products:

• GAFX-IG - Goat anti-human Factor X, whole IgG from antiserum
• GAFX-AP - Goat anti-human Factor X, affinity purified IgG
• GAFX-HRP - Goat anti-human Factor X, peroxidase labelled IgG
• SAFX-IG - Sheep anti-human Factor X, IgG from antiserum
• SAFX-AP - Sheep anti-human Factor X, affinity purified IgG
• RAFX-HRP - Rabbit anti-human Factor X, peroxidase labelled IgG
• FX-DP - Human plasma deficient in Factor X, immune depleted