DiaPharma Group, Inc.

For Research Use Only. Not For Use In Diagnostic Procedures.

Vial containing whole IgG conjugated to horseradish peroxidase (HRP) through carbohydrate groups. Total protein is 0.2 mg. Suitable as a source of peroxidase-labeled antibodies to human Tissue Plasminogen Activator (tPA).

Format: IgG-HRP conjugate as a clear, slightly red-brown liquid.

Host Animal: Sheep

Immunogen: Human tissue-type plasminogen activator prepared from melanoma cell line.

Concentration: IgG-HRP concentration is determined by absorbance using an extinction coefficient (E^1%₂₈₀) of 14.

Buffer: A buffered stabilizer solution containing 50% (v/v) glycerol.

Storage: Store between -10 and -20°C. Product will become viscous but will not freeze. Avoid storage in frost-free freezers. Keep vial tightly capped. Allow product to warm to room temperature and gently mix before use. Avoid exposure to sodium azide as this is an inhibitor of peroxidase activity.

Specificity: Prior to conjugation, this antibody was specific for tPA as demonstrated by immunoelectrophoresis and ELISA.

Rz Ratio (Reinheitszahl, A403/A280): determined spectrophotometrically.

Related Products:

• SATPA-IG - Sheep anti-human tPA, whole IgG from antiserum
• SATPA-AP - Sheep anti-human tPA, affinity purified IgG
• TPA-EIA - Paired antibody set for ELISA of tPA, 5 x 96 tests