DiaPharma Group, Inc.

For research use only.

S-2288 is a chromogenic substrate sensitive to a broad spectrum of serine proteases. The substrate has been used for the determination of Tissue Plasminogen Activator in purified preparations.

Reagent -- Composition, Packaging, Storage and Stability

Each vial contains chromogenic substrate S-2288, 25 mg and mannitol 40 mg as a bulking agent. Stability: Substance: Stable until expiry date if stored at 2-8°C. Avoid exposure to light. The substance is hygroscopic and should be stored in a dry place. Solution: 10 mmol/L in H2O is stable for more than two months at 2-8°C.

** Also available in bulk sizes (500+ mg) as a special order.

Chemistry

Chemical name: H-D-Isoleucyl-L-prolyl-L-arginine-pnitroaniline dihydrochloride.

S-2288 structure

Formula: H-D-lle-Pro-Arg-pNA · 2HCl

Mol. wt: 577.6

e316 nm: 1.27 · 104 mol-1 · L · cm-1

Solubility: > 40 mmol/L in H2O

Suitable stock solution: 10 mmol/L in H2O

Kinetic Data

See package insert for kinetic data.

Standardization

The tissue plasminogen activator is not affected by the following inhibitors used at the concentrations given.

1. Trasylol 30 KIU/mL.
2. Soybean trypsin inhibitor 50 mg/mL.
3. Antithrombin 0.1 PEU/mL and heparin 3 IU/mL.