DiaPharma Group, Inc.

For In Vitro Research Use Only.

The Technozym ADAMTS-13 INH ELISA is a test for detection of human autoantibodies (IgG) in serum or plasma against ADAMTS-13, the protease which is responsible for cleaving unusually large vWF (ULvWF). Most of these autoantibodies inhibit ADAMTS-13 activity and thus non-cleaved ULvWF accumulates in plasma. This is believed to be the major cause for thrombotic thrombocytopenic purpura (TTP). TechnozymADAMTS-13 INH test makes it possible to differentiate between congenital (gene polymorphisms) and acquired (autoantibodies) TTP when coupled to an activity assay and to control efficacy of plasma exchange therapy.

Composition

1. ELISA test strips (6), with 8 wells each, coated with a recombinant form of ADAMTS-13 protease; the drying agent is supplied in an aluminium bag.
2. Washing buffer concentrate (PBS; pH 7.3); containing detergent; 0.01% merthiolate; 1 vial, 100 mL.
3. Incubation buffer (= sample dilution buffer) (PBS; pH 7.3); contains stabiliser protein; 0.01% merthiolate; and dye, 1 vial, 100 mL, ready for use.
4. Calibrators (Standards) numbered from 1 to 5; lyophilised; 1 vial each; 0.5 mL. Concentrations are lot-specific; consult the label on the vial.
5. Positive and negative control plasma; lyophilised; 1 vial each; 0.5mL. Concentrations are lot-specific; consult the label on the vial.
6. Conjugate: anti-human IgG POX; dyed blue; 1 vial, 0.3 mL.
7. Chromogenic substrate TMB (tetramethylbenzidine); 1 vial; 12 mL; ready to use.
8. Stopping solution sulphuric acid 1.9 mol/L; 1 vial; 12 mL; ready to use.
9. Adhesive film: for ELISA test strips; 2 pieces.