DiaPharma Group, Inc.

For In Vitro Research Use Only.

The Technozym ADAMTS-13 ELISA is a test for the determination of ADAMTS-13 activity and antigen concentration in human plasma. ADAMTS-13 is the enzyme that cleaves vWF under laminar flow conditions. A functional defect of this enzyme leads to the presence of higher molecular weight forms of vWF and thus to increased platelet aggregation, mainly in the microvasculature. This is believed to be the major cause for thrombotic thrombocytopenic purpura (TTP).

COMPOSITION

1. ELISA test strips (2x6 for 2x48T), with 8 wells each, coated with a monoclonal anti ADAMTS13, directed against the CUB domain; the drying agent is supplied in an aluminium bag.
2. Washing buffer concentrate (PBS; pH 7.3); containing detergent; 0.01% merthiolate; 1 vial, 100 mL.
3. Incubation buffer (PBS; pH 7.3); contains stabiliser protein; 0.01% merthiolate; and dye, 1 vial, 100 mL, ready for use.
4. Calibrators (Standards) numbered from 1 to 5; lyophilised; 1 vial each; 0.5 mL. Concentrations are lot-specific; consult the label on the vial or the included batch table.
5. High and low control plasma; lyophilised; 1 vial each; 0.5mL. Concentrations are lot-specific; consult the label on the vial.
6. Activity substrate; 2 vials for 2x48T.; 3 mL each, lyophilised.
7. Conjugate: anti-ADAMTS-13 POX; dyed blue; 1 vial, 0.3 mL.
8. Antigen substrate: 1 vial, 6 mL.
9. Stable peroxide solution; 1 vial, 0.7mL.
10. Stop solution for antigen substrate: 1 vial; 6 mL; ready to use.
11. Adhesive film: for ELISA test strips; 2 pieces.