ADAMTS-13

Microplate Reader flash luminescence assay fluorescence coagulation analyzer

BIO-TEK FLx 800 TBI Fluorometer

Instrument

Catalog #: 9810010

For assaying thrombin generation using TechnothrombinTGA you need a fluorescence reader equipped with filters 360 nm and 460 nm (excitation/emission) as the BIO-TEK FLx 800 TBI with an adapted software for TechnothrombinTGA.

The FLx800 is a multi-detection microplate reader that combines excellent specifications and performance with convenience and ease of use. This design incorporates powerful performance at a price much lower than traditional fluorescence-luminescence microplate readers. The FLx800 line includes several models with options that meet the specific needs of research and OEM users. Top and bottom reading are available to read anything from 6-well to 384-well microplates, and PCR plates. An optional external dispenser module allows to run applications such as ion channel assays and flash luminescence assays.

Features

fluorescence reader microplate fluorometer coagulation analyzer hemostasis thrombosis

The FLx800's convenient front panel access makes changing filters and lamps and probe positioning quick and easy.

  • Custom-mapped fiber optics for optimal signal transfer
  • Selectable, automated top- or bottom-probe reading for best sensitivity
  • Typical fluorescence sensitivity to 5 pg sodium fluorescein
  • Glow luminescence reading with top detection for best sensitivity
  • Incubation to 50ºC for temperature-sensitive assays
  • PCR tube-reading option available
  • Reads 6- to 384-well formats and PCR plates
  • Convenient front panel accessory access makes changing filters simple
  • Powerful on-board data reduction software
  • Computer control

Specifications

Detection method

Fluorescence, Luminescence:
Low Noise Photomultiplier tube

Read method

Endpoint, kinetic and area scanning under computer control

Microplate types

6- to 384-well plates, PCR plates (FLx800TBP)

Reading speed

Kinetic, full 96-well plate in <35 seconds (depends on settings)

Wavelength range

Excitation: 300-650 nm
Emission: 300-700 nm
(300-800 nm with optional PMT)

Wavelength selection

Deep blocking interference filters

Filter wheel capacity

Excitation: 4 positions, Emission: 4 positions

Filters supplied

2 excitation and 2 emission filters

Bandpass

Variable, dependent on filter

Dynamic range

0-99.999 RFUs
0-99.999 RLUs

Sensitivity

Sodium fluorescein 2.5 pg/ml SF (1.3 fmol/well) typical*
Luciferase 0.1 pg/well typical
ATP 100 amol/well limit of detection
(*Black plate, top measurement, 3mm diameter probe)

Standard probes

3.0 mm top
5.0 mm bottom

Light source

Tungsten Halogen, 1000 hours lifetime

Temperature control

4oC above ambient to 50oC (I models)

Shaking

Linear shaking (I models)

Reagent dispensers

1 syringe pump

Dispense volume

5-1000 microliters in 1 microliters increments

Onboard software

55 user-programmable protocols

PC Software

Gen5TM (optional)

Power

100-240 VAC 50/60 Hz

Dimensions

15" W x 16" D x 9" H
(38.1 x 40.64 x 22.89 cm)

Weight

30 lbs (13.6 kg)

Regulatory

For in-vitro diagnostic use (Except in the European Union).
All BioTek microplate instrumentation is CE and ETL marked. For details on approvals and standards compliance, please contact BioTek.

Accessories Included

  • 2 Excitation filters (485/20, 360/40)
  • 2 Emission filters (528/20, 460/40)
  • Serial cable: null modem, 9-pin female to 25-pin female
  • Operator's manual

Downloads

Name Type Size
BioTek FLx800 Manual application/pdf 25.00 MB
BioTek FLx800 Microplate Reader Flyer application/pdf 153.89 kB
DiaPharma Product Range (Technoclone) application/pdf 329.44 kB