DiaPharma Group, Inc.

Research ELISA for determination of von Willebrand Factor collagen binding activity. In combination of von Willebrand disease type 1, 2 and 3 is possible.

INTENDED USE

The von Willebrand Factor (vWF) is a large, multifunctional glycoprotein, occupying a key position in primary haemostasis. It has a multiple structure with several functions:

• It is the carrier protein for Factor VIII in plasma; it forms a complex and thus protects Factor VIII from early proteolytic decomposition.
• It acts as a mediator for platelet aggregation by attaching itself to platelet membrane receptors (GP Ib and GP lIb/IIIa) following previous platelet activation.
• It plays a part in primary hemostasis by acting as a mediator between adhesioned platelets and the subendothelium (lesioned vascular wall).

In order to analyze the adhesive properties, as a rule the platelet aggregation is measured (measuring system = ristocetin-dependent platelet aggregation). However, this does not reflect the physiological setting nor the function of the vWF. For determining the adhesive properties of the vWF, its binding capacity to collagen serves as a parameter which corresponds to the physiological function of the vWF.

COMPOSITION

1. ELISA test strips (12) with 8 wells each, coated with human collagen Typ III; the drying
   agent is supplied in an aluminum bag.
2. Washing buffer concentrate: (PBS; pH 7.3); containing detergent; 0.01% merthiolate; 1
   bottle, 100 mL.
3. Incubation buffer: (PBS; pH 7.3); contains stabilizer protein; 0.01% merthiolate; and dye 1
   bottle, 100 mL, ready for use.
4. Calibrators (Standards) numbered; lyophilized; 1 bottle each.
   Concentrations are lot-dependent; consult label on the vial.
5. Control plasmas "low level" and "high level" for checking purposes, lyophilized; 1 bottle
   each. Concentrations are lot-dependent; consult the label on the vial.
6. Conjugate polyclonal Anti-vWF-POX; dyed blue; 1 bottle, 0.3 mL.
7. Chromogen TMB (tetramethylbenzidine); 1 bottle, 12 mL; ready to use.
8. Stopping solution: sulphuric acid 1.9 mol/L; 1 bottle 12 mL; ready for use.
9. Adhesive film: for ELISA test strips (2).