Activated Protein C (APC)

Can I freeze the reagents in the Coatest APC resistance kits to prolong their stability?

The APTT reagent cannot be frozen. The other reagents can, but should be rapidly thawed at 3...

Can I use Coatest APC Resistance V without the predilution with FV deficient plasma? Isn’t this just like using the APC R kit?

No. There is more APC in the APC V kit than in the classic kit. This makes the APC ratios tw...

Describe the interaction between Protein S and APC.

Only the free, native form of PS binds to APC and functions as a cofactor. PS has the highes...

Describe the protein C pathway and APC resistance. What is the relative risk of venous thrombosis for those who are APC resistant?

Protein C is a vitamin-K-dependent glycoprotein and plasma proenzyme of a serine protease th...

Explain the types of protein C deficiency, and the clinical manifestations.

Hereditary protein C deficiency is inherited as an autosomal dominant trait. Heterozygotes f...

How do factor V and VIII levels affect the Coatest APC Resistance tests?

A study by Colucci et al. (Thromb. Haemost. 1994, 72:987 - 988) using the classical APTT met...

What effect does variation of plasma levels of protein C have on the APC ratio?

Variations in plasma levels of protein C have no influence on the APC ratio since a standard...

What is the normal baseline range for the APTT reaction? What types of conditions might cause a patient to give abnormal aPT time, and what can be done about it?

The normal range is approximately 25-40 seconds, although it will vary slightly depending on...

Which assay should I run – Coatest APC Resistance or Coatest APC Resistance V?

During the last years recent publication have been showing that the APC Resistance phenotype...