Antithrombin (AT)

Can Coamatic Antithrombin be used to measure mouse plasma?

Yes.

Coamatic Heparin is optimized for use with and without exogenous antithrombin. When is it recommended to add exogenous AT, and why?

It is recommended to add exogenous antithrombin when children below the age of one year are...

Describe heparin’s interaction with antithrombin. Why is the anti-FXa method a better way to measure heparin activity?

Slow protease-antithrombin interactions are enhanced dramatically in the presence of certain...

Even though there is documentation that the anti-FXa method is better, how could a researcher determine antithrombin in plasma based on a thrombin method?

A thrombin-based chromogenic heparin cofactor assay for the determination of antithrombin ac...

Explain the function of antithrombin and its interaction with thrombin and heparin?

Antithrombin is the most important natural inhibitor of the coagulation cascade, accounting...

Explain the types of antithrombin deficiency, and the clinical manifestations. What treatment options are available?

A normal AT range is assumed to be 80-120%. Individuals with low antithrombin levels have an...

How should I make my standard pre-dilutions? It is not very clear from the package insert.

A suggested method for the predilution, although it cab be up to the analyst what volumes to...

I am getting lower levels of antithrombin than expected. What could I be doing wrong?

In plasma where contact activation has occurred, a contribution to the substrate activity mi...

What is the conversion factor for Antithrombin from IU to mg?

5 IU corresponds to 1 mg. Therefore a 25 IU vial corresponds to 5 mg AT. The molecular weigh...

What is the difference between Coamatic Antithrombin and Coamatic AT 400 and Coamatic Antithrombin LR?

These kits use substrate S-2772. Coamatic AT 400 has been developed for use with undiluted p...

When exogenous AT is desired, how should it be added in the Coamatic Heparin test?

Add to the assay an equal volume of 1 IU/ml AT as the plasma volume. AT, 10 IU, can be bough...

Why did Chromogenix discontinue Coatest Antithrombin and replace it with Coamatic Antithrombin?

Papers have documented that thrombin-based AT assays, such as Coatest AT, show an overestima...