Bioreagents

Can the tPA activity standard be used to measure two-chain tPA or just single chain tPA?

The t-PA 10 mg activity standard is composed 95% of single-chain t-PA with a specific fibrin...

Define a katal.

One katal (kat) is the amount of enzyme that converts one mole of substrate per second. Acti...

Define an enzyme and a substrate.

Enzymes are proteins that catalyze most of the chemical reactions that take place in the bod...

How can I measure prothrombin activity? What is considered an elevated level of prothrombin activity?

A mutation G ® A in the untranslated 3? region of the prothrombin gene at nucleotide positi...

Is the plasminogen reagent Glu- or Lys- plasminogen?

It is a preparation of at least 95% Glu-plasminogen.

The activity of plasmin and plasminogen is expressed in CU. What is this, and what is the equivalent in nkat?

CU stands for Casein Units, and is a measure of the proteolytic activity on the substrate ca...

The factor Xa reagent says it is 71 nkat, but I need to know what that is in g/ml and mol/ml.

One katal (kat) is the amount of enzyme that converts one mole of substrate per second. Acti...

The majority of the Chromogenix substrate library has an Arginine (Arg or R) group at the P1 position (the amino acid position that occurs at the preferred cleavage site). Why is this?

The Chromogenix line is geared toward the proteins involved in hemostasis. These are a group...

What is the 53 nkat vial of thrombin equivalent to in NIH units?

Based on an assay with S-2238, for bovine thrombin,1 NIH-U = 1.15 IU = 3.4 nkat. According t...