Species Cross-Reactivity - Using paired antibodies for EIA

The values given are the reactivities relative to human reference plasma (W.H.O. traceable) when the test plasmas are assayed under the conditions specified in the protocol for each assay. Plasmas with reactivities of less than 1% are listed as negative. Optimization of the assay conditions may be required to efficiently utilize these assays for the measurement of these analytes in animal plasmas. Below are examples of typical results seen in historical lots.

 

Analyte

Human

Rat

Mouse

Rabbit

FG-EIA

Fibrinogen antigen

100%

66%

18%

NT

RBFG-EIA

Rabbit Fibrinogen antigen

47%

19%

19%

NT

VWF-EIA

von Willebrand Factor antigen

100%

15%

6%

34%

FII-EIA

Prothrombin antigen

100%

neg

neg

17%

F8C-EIA

Factor VIII antigen

100%

neg

neg

neg

FIX-EIA

Factor IX antigen

100%

neg

neg

14%

FX-EIA

Factor X antigen

100%

neg

1%

neg

PC-EIA

Protein C antigen

100%

neg

neg

neg

PS-EIA

Protein S antigen

100%

neg

neg

2%

ATIII-EIA

Antithrombin antigen

100%

13%

25%

neg

PG-EIA

Plasminogen antigen

100%

4%

5%

11%

TAFI-EIA

Thrombin Activatable Fibrinolysis Inhibitor

100%

3%

7%

neg

Legend: neg = negative NT = not tested

 

Analyte

Canine

Feline

Porcine

Bovine

FG-EIA

Fibrinogen antigen

30%

44%

14%

neg

RBFG-EIA

Rabbit Fibrinogen antigen

28%

26%

14%

17%

VWF-EIA

von Willebrand Factor antigen

31%

NT

31%

neg

FII-EIA

Prothrombin antigen

neg

neg

neg

neg

F8C-EIA

Factor VIII antigen

neg

NT

neg

neg

FIX-EIA

Factor IX antigen

1%

NT

neg

neg

FX-EIA

Factor X antigen

1%

NT

neg

neg

PC-EIA

Protein C antigen

neg

neg

neg

neg

PS-EIA

Protein S antigen

neg

2%

neg

neg

ATIII-EIA

Antithrombin antigen

9%

21%

4%

neg

PG-EIA

Plasminogen antigen

33%

NT

NT

3%

TAFI-EIA

Thrombin Activatable Fibrinolysis Inhibitor

neg

NT

5%

neg

Legend: neg = negative NT = not tested